Determination of malondialdehyde (MDA) level of plasma and lung tissue

Hamed Ghavimi; Sasan Sheidaei; Haleh Vaez; Elmira Zolali; Parina Asgharian; Hadi Hamishehkar

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Determination of malondialdehyde (MDA) level of plasma and lung tissue

HG Hamed Ghavimi

SS Sasan Sheidaei

HV Haleh Vaez

EZ Elmira Zolali

PA Parina Asgharian

HH Hadi Hamishehkar

This method is extracted from research article: Iran J Basic Med Sci, May 2018

Metformin-attenuated sepsis-induced oxidative damages: a novel role for metformin

DOI: 10.22038/IJBMS.2018.24610.6126

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MDA, which is the main by-product of polyunsaturated fatty acid oxidation, is a known biomarker of lipid peroxidation principally induced by ROS. The MDA concentration in plasma and lung tissue was determined using the Thiobarbituric Acid-Reactive Substance (TBARS) assay. In this method, MDA reacts with thiobarbituric acid to produce a pink color and its absorbance is measured spectrophotometrically at 532 nm. The MDA level was expressed as nmol/ml tissue extract normalized to protein content of the sample (nmol/ml tissue extract/mg protein) for tissue MDA and nmol/ml of plasma for plasma MDA (26).

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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