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Immunoblotting

NH Nicole A. Hawkins
NZ Nicole J. Zachwieja
AM Alison R. Miller
LA Lyndsey L. Anderson
JK Jennifer A. Kearney
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Forebrain membrane proteins were isolated from P24 ISC-D mice carrying homozygous B6, homozygous 129, or heterozygous alleles. Membrane fractions (50 μg/lane) were separated on a 7.5% SDS-PAGE gel and transferred to nitrocellulose membranes. Immunoblots were probed for GABRA2 using a rabbit polyclonal antibody (Phosphosolutions, 822-GA2C) and for Mortalin using a mouse monoclonal antibody (NeuroMab, 75–127). Alexa-conjugated fluorescent secondary antibodies (Jackson ImmunoResearch) were used to detect bound primary antibody using an Odyssey imaging system (Licor).

Copyright and License information:  ©2016 Hawkins et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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