C. elegans rescue experiments-transgenics

Hco-acr-8 coding sequence was sub-cloned into the pPD96.52 (Addgene) vector containing a myosin promoter Pmyo3, using the In-FusionHD cloning kit (Clontech). Primer sequences are listed in S2 Table. C. elegans lev-8 (ok1519) were injected with 30ng μl^-1 of the resulting plasmid to drive the expression of H. contortus acr-8 in the body muscle cells. Transformed worms were identified by co-injecting pPD118.33 (Pmyo-2::gfp) plasmid at 50ng μl^-1 (Fire lab vector kit), which drives expression of green fluorescent protein (GFP) from the pharyngeal muscle promoter Pmyo-2. The co-injected gfp transformation marker forms an extra-chromosomal array with the plasmids carrying the gene sequence and thus worms with fluorescent green pharyngeal muscle can be identified as carrying the plasmid of interest. For all the experiments, two independently transformed stable lines of transgenic C. elegans were assayed.