HPLC analysis of phenolic compounds

The phenolic profile of fig extract was quantified using an Agilent 1260 HPLC system (Agilent Technologies, Germany) with the basic parts like degasser, auto-sampler, quaternary pump and diode array detector (DAD). For phenolic analysis, the separation was achieved with a Zorbax Eclipse C18 column. The mobile phase consisted of solvent A (methanol: acetic acid:  water:  10: 2: 88 v/v), and B (methanol: acetic acid: water 90: 2: 8 v/v) with the elution program as reported previously (Zeb 2015b). Elution time was 25 min. Phenolic compounds were analyzed using diode array detector at 280, 320 and 360 nm. Absorption spectra were recorded in 190–500 nm. Identification of phenolic compounds was based on the standard calibration curves of p-hydroxybenzoic acid, caffeic acid, caftaric acid, cichoric acid, and quercetin-3-glucoside and also tentatively done by comparing the absorption spectra with those in the literature.