0.0256 μg of palmitic acid was added to 1000 μL of 99% ethanol and heat at 50°C for 3 min. 1.0g of BSA was dissolved in 9ml water and 40 μL of 1N NaOH, heated at 50°C for 3 min. 100 μL of palmitic acid solution were added in 900μL of BSA solution.
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