2.3. Cell Culture of HT29

HT29 cells are human colon adenocarcinoma cell line and repeatedly is used as a model of luminal surface colonic epithelial cells. We purchased HT29 from the Cell Bank at the International genetic and biological center. Cells were cultured in RPMI enriched with 10% FBS, 20mM HEPES and 1% L-glutamine and 100U/ml penicillin, 100µg/ml streptomycin. Cell culture medium was renewed every 1-2 days for 4-5 days until when the full polarization of the HT-29 cell monolayer was achieved. One million HT29 cells were divided into each well of 12-well cell culture plates.