Determination of anti-inflammatory activity using rat paw oedema model

Anti-inflammatory activity of the ethyl acetate extract was carried out according to the method of Okokon et al. (2012) and Kim et al. (2017) with slight modification in doses and time. A total of 168-week old Swiss albino rat models (equal number of males and females) were grouped into 4 groups of 4 rats per group. Group 1 received 10 mg/kg of distilled water, group 2 received 1.66 mg/kg of diclofenac [dose determined using km factor calculation (Shin et al. 2010)], group 3 received 250 mg/kg of extract, group 4 received 500 mg/kg of extract. One hour after the administration, inflammation was induced by single sub-plantar injection of 0.1 mL raw egg albumin, then inflammation was checked at 30 min, 1, 2, 3 and 4 h post administration for the paw size. The % inhibition was calculated using the following formula:

where A = Difference in control (Sterile water group), B = Difference in treatment group.