2.5. Dynamic Light Scattering (DLS) Analysis and ζ Potential Measurements

The mean diameter, width of distribution (polydispersity index, PDI), and ζ potential of the nanoparticles were measured at 25 °C using a Zetasizer NanoZS instrument (Worcestershire, UK) fitted with a 532 nm laser at fixed scattering angle of 173°. The intensity-average hydrodynamic diameter (size in nm) and PDI of nanosystems were characterized in double distilled water after 2 and 20 days. Polymer dispersions were obtained at room temperature by stirring samples (0.2–2 mg/mL) until complete dispersion (2 days). The ζ potential (mV) was calculated from the electrophoretic mobility using the Smoluchowsky relationship and assuming that K·a >> 1 (where K and a are the Debye-Hückel parameter and particle radius, respectively). Each experiment was performed in triplicate.