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293 T cells were plated at 60-70% confluence in 24-well plates and transiently transfected with 1 μg of different fragments of CCL2 promoter-connected luciferase reporter constructs together with expression plasmids for STAT3 using FuGENE 6 transfection reagent (Roche Applied Science). Empty pcDNA3.1 vector was used as a control. Firefly luciferase activity was normalized to that of Renilla luciferase, which were cotransfected under the control of the SV40 early enhancer/promoter region (pSV40-RL, Promega).
Copyright and License information: ©2019 JunLi Xue et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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