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Matrigel invasion assays

CW Chaoyu Wang
XZ Xin Zhu
CP Chibin Pu
XS Xuan Song
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Colorectal cell invasiveness was determined in a 24-well transwell plate (8 µp pore size; Costar), as described previously (26). Briefly, 5×104 cells were placed in the upper chamber of each insert coated with 200 mg/ml of Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). After 48 h, the invaded cells were stained with haematoxylin and counted. Each experiment was repeated three times independently.

Copyright and License information:  ©2018-03-01 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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