2.5. MTT Assay

For the MTT assays, 3-[4,5-dimethylthiaoly]-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St. Louis, MO, USA) was used. Briefly, OVCAR3 and OVCAR8 cells (50 × 10³ cells/well) were seeded into a 24-well plate. Upon specific treatments, fresh MTT 2 mg/mL (Sigma-Aldrich, St. Louis, MO, USA), resuspended in PBS, was added to each well containing both the cell lines; fresh MTT 2 mg/mL (Sigma-Aldrich, St. Louis, MO, USA). After 2 h incubation, culture medium was discarded and replaced with 200 μL of isopropanol. Optical density was measured at 595 nm in a spectrophotometer. Analysis of OVCAR3 and OVCAR8 cell growth was performed at 0 h, 12 h, 24 h, 48 h and 72 h. For each sample, MTT assay was performed in triplicate.