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2.2. Quantification of melanin content in vitro
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Melanin content of cultured cells was measured as described.15, 16 Briefly, medium was replaced, cells were cultured 48 hours and cells were then homogenized in 3N NaOH (Wako). Melanin content of homogenates was determined by measuring absorbance at 405 nm using a plate reader (ARVO™ X3; PerkinElmer, Waltham, MA, USA).
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