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Measurement of pro-inflammatory cytokines (TNF-α and IL-6) production
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The inhibitory effect of WE(2-1) and AL(2-1) on the production of proinflammatory cytokines (TNF-α and IL-6) from LPS-treated RAW 264.7 cells was determined. The supernatants were subsequently employed for the proinflammatory cytokine assays using a mouse enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA).
Copyright and License information: ©2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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