2.7. Mouse Xenograft Studies

Mice were purchased from Charles Rivers Labs and surgically castrated before cell inoculations under the skin. We used NTsh (control for KD), CPT1A-KD, EV (control for OE) and CPT1A-OE cell lines from C4-2 and 22Rv1 models to generate the xenografts. NSG mice were used for the C4-2, and nu/nu mice for 22Rv1 cells. About 2 × 10⁶ cells were inoculated per flank, and tumor growth was monitored daily with calipers. For MDV3100 (enzalutamide or Enza) studies, tumors were generated the same way, and when they reached ~500 mm³, we initiated oral treatment with Enza at 20 mg/Kg every other day for 3 weeks. Tumor growth was calculated using the formula volume = (π × length × width²) / 6.