Nomenclature of pTRA plasmids

We recommend a systematic nomenclature, which contains all genetic components that are added to the pTRA cargo site. To shorten the plasmid names, we chose to number all used promoters, genes, and regulatory sites. Our nomenclature is based the style pTRA_Promoter_d_h_GOI_h_d, while d stands for dBroccoli-tag and h for his-tag. Unused features can be entitled as ‘0’. For example, the plasmid pTRA-51hd used in this work, is composed of the promoter ‘5’ (XylS/Pm), the gene of interest ‘1’ (mCherry), and the 3’-end tagging of the mRNA ‘d’ (dBroccoli-tag) as well as a C-terminal fusion ‘h’ (his-tag).