Mosquito eggs of Ae. aegypti were collected in September 2017 from ovitraps placed in different locations of Gómez Palacio, Durango, Mexico. Eggs were hatched and reared to the fourth instar stage. A portion of fourth instar larvae and pupae were killed in hot water (60-80 °C), while a portion of the adult females was killed in lethal chambers using chloroform vapors. All specimens were identified to species and immediately transferred to labeled vials with 90% alcohol and frozen at -40 °C until analysis.
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