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Isolation and culture of primary colon epithelial cells

XZ Xudong Zhu
WS Weiyan Shen
YW Ying Wang
AJ Amit Jaiswal
ZJ Zhenyu Ju
QS Qinsong Sheng
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Primary colon epithelial cells were isolated from freshly excised colon tissue following collagenase type A (Roche, Basel, Switzerland) digestion. Dulbecco’s modified Eagle’s medium (HyClone, Logan, UT, USA) containing 4.5 g l−1 glucose, penicillin–streptomycin (100 IU ml−1 to 100 μg ml−1) and 10% foetal bovine serum (HyClone) was used for primary cell culture.

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