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High performance liquid chromatography (HPLC)

MK Meihua Christina Kuang
PH Paul D Hutchins
JR Jason D Russell
JC Joshua J Coon
CH Chris Todd Hittinger
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The pre-culture conditions were identical to those described above for the growth assays. At indicated time points, 1 mL of cells were centrifuged, and 500 μL supernatant was harvested and frozen at −80˚C. HPLC was conducted at the GLBRC Metabolomics Lab using an HPLC-RID system with an Aminex HPX-87H (BioRad, Inc. Hercules, CA) following previously described protocols (Moore and Johnson, 1967; Ehrman and Himmel, 1994). Instrument control, data collection and analyses were conducted using ChemStation B.04.03 software (Agilent Technologies, Inc., Palo Alto, CA).

Copyright and License information:  ©2016, Kuang et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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