The Caspase-Glo 3/7 assay (Promega, Madison, WI) was used according to the manufacturer’s protocol. Cells were assayed between 9 and 12 days of ex vivo expansion. Thereafter, 4 μM camptothecin was added to cells for 2.5 hours as a positive control.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.