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Expression of interferon stimulated genes
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Naïve Huh7 cells were cultured in the presence of 5 μg/mL MPA in combination with 0.1 nmol/L DCV or 10 nmol/L ASV. DMSO was used as a vehicle control. After 48 h RNA was isolated and quantified and cDNA was synthesized. The levels of Interferon regulatory factor 1 (IRF1), Interferon regulatory factor 9 (IRF9), and Interferon-induced transmembrane protein 3 (IFITM3), with GAPDH as a reference gene, were quantified with RT-qPCR using SYBR green.
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