2.6. Cell proliferation assay

Cell growth was further confirmed with CellTiter 96® Aqueous One Solution Reagent (Promega) following the manufacturer's instructions. Either HSC-3 or RSC-96 cells were seeded at a density of 10k cells/well in 96-well plates. After 24 hours in culture, cell inserts for 96-well plates (3-μm pore size, xCELLigence Systems) or drugs were added into each well. Cells were incubated for another 48 hours until cell proliferation was measured at 490 nm optical density (OD) using a microplate reader (Promega). For co-culture experiments, the same density of HSC-3 (10k) and RSC-96 (10k) cells was used (Fig. 2A, Fig. 3A). Experiments were performed in triplicates and were repeated twice.