2.10. CCK-8 Cell Viability Assay

The HepG2 cells were seeded in 96-well plates at a density of 5000 cells per well, and the cells were cultured in the indicated culture medium, supplemented with lentiviral particles of RORγ overexpression and control vector. The cells were cultured for a different time, and before 3 h of indicated detection time, 10 μL CCK-8 reagent (Yeasen, Shanghai, China) was added into the cells, and the absorbance value was detected by a microplate reader (Thermo Scientific, Madison, WI, USA) at 450 nm.