RPPA

Cells were lysed and prepared as described previously (Tibes et al, 2006) and RPPA performed at the MD Anderson Functional Proteomic core facility (Houston, TX). RPPA data were determined and analyzed as described (Cheng et al, 2017). Comparisons were performed between conditioned samples using the two‐sample t‐test method with 1,000 permutations. Multiple hypothesis test corrections were calculated, and antibodies with a Storey q‐value < 0.05 and a fold ratio > 2 were considered significant, unless noted otherwise. Calculations were performed in MATLAB^® (v2017b) using the mattest and mafdr functions.