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Real-time RT-PCR

RT Reshef Tal
SS Shafiq Shaikh
PP Pallavi Pallavi
AT Aya Tal
FL Francesc López-Giráldez
FL Fang Lyu
YF Yuan-Yuan Fang
SC Shruti Chinchanikar
YL Ying Liu
HK Harvey J. Kliman
MI Myles Alderman, III
NP Nicola Pluchino
JK Jehanzeb Kayani
RM Ramanaiah Mamillapalli
DK Diane S. Krause
HT Hugh S. Taylor
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For each sample, 500 ng of total RNA was reverse transcribed using an iScript cDNA Synthesis Kit (Bio-Rad, Wallingford, CT) according to the manufacturer’s instructions. qPCR was performed using iQ SYBR Green Supermix (Bio-Rad) on a Bio-Rad CFX96 thermocycler using the gene-specific primer pairs listed in S2 Table. Gene expression was analyzed on duplicate samples and the Ct values were normalized to the GAPDH housekeeping gene.

Copyright and License information:  ©2019 Tal et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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