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The HT29 and SW480 cells were transfected with miR-control, miR-205-5p, miR-205-5p and vector, and miR-205-5p and PTK7, respectively, for 72 h. The treated HT29 and SW480 cells were incubated in complete medium for 14 days. The colonies were fixed with methanol for 15 min at room temperature, and dyed with giemsa dye solution for 10 min at room temperature. The colonies were then identified and counted under a light microscope (BX51; Olympus Corporation, Tokyo, Japan).
Copyright and License information: ©2018 Chen et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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