Supplemental Data

The following supplemental materials are available:

Supplemental Figure S1. Hypocotyl phenotype and length in various BBX30 and BBX31 transgenic seedlings grown in darkness.

Supplemental Figure S2. Light does not affect the accumulation of YFP-BBX30 or YFP-BBX31.

Supplemental Figure S3. Mutations in each of the two independent bbx30 and bbx31 alleles created by the CRISPER/Cas9 method.

Supplemental Figure S4. Hypocotyl phenotype and length in bbx30, bbx31, and bbx30 bbx31 seedlings grown in darkness.

Supplemental Figure S5. Validation of 18 target genes of BBX30 and BBX31 identified by RNA-Seq using RT-qPCR.

Supplemental Figure S6. Hypocotyl phenotype and length in hy5-215 bbx30-2, hy5-215 bbx31-2, and hy5-215 bbx30-2 bbx31-2 grown in darkness.

Supplemental Figure S7. bbx30-2 bbx31-2 display earlier flowering phenotype, but BBX30 and BBX31 transgenic plants show delayed flowering phenotype under long-day conditions.

Supplemental Figure S8. BBX30^D46A and BBX31^D45A gene and protein expression levels in each of two independent transgenic lines.

Supplemental Figure S9. Hypocotyl phenotype and length in BBX30^D46A and BBX31^D45A transgenic seedlings grown in darkness.

Supplemental Table S1. List of up- and down-regulated gene in the bbx30-2 bbx31-2 double mutant compared with the wild type.

Supplemental Table S2. Enriched GO terms (biological process) and genes of the down-regulated genes in the bbx30-2 bbx31-2 double mutant.

Supplemental Table S3. Enriched KEGG pathways and genes of the down-regulated genes in the bbx30-2 bbx31-2 double mutant.

Supplemental Table S4. List of primers used in this study.