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To investigate the migration ability of HT29 cells, HT29 cells were seeded on six-well culture plates at a density of 1 × 105 cells per well and allowed to grow to confluence over 24 h. The confluent monolayers were scratched with a pipette tip and cultured for 5 days. The plates were washed once with medium to remove the nonadherent cells and photographed under a phase-contrast microscope.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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