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Progesterone ELISA assay
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The progesterone concentration in serum-free medium from primary bovine granulosa cells was measured after 48 h of treatment using ELISA assays [37]. The limit of detection for progesterone was 12 pg/tube. The intra- and inter-assay coefficients of variation for progesterone were less than 10% and 11%, respectively. The results were expressed as the concentration of progesterone (ng/mL) per mg of protein per well. Data were obtained from three independent cultures, and each treatment (recombinant human CCL21: 10, 50, 100, 200 ng/mL) had four replicates.
Copyright and License information: ©2019 Mellouk et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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