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The Glow-Substrate peptide was unfolded by heating at 40 °C for 7 min and subjected to a refolding assay using the HSP70/HSP40 Glow-Fold Protein Refolding kit (BostonBiochem) according to the manufacturer’s instructions. For the quantification of the refolded amounts of peptide, the luciferase signal was measured by a luciferase reader (GloMax, Promega) at various reaction times (10, 20, 30, 40, 50, and 60 min). The experiments were independently repeated three times.
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