Animal surgery

JS Jiaojiao Song
DS Da Shao
XG Xinli Guo
YZ Yanfang Zhao
DC Dongyang Cui
QM Qianqian Ma
HS Huan Sheng
LM Lan Ma
BL Bin Lai
MC Ming Chen
PZ Ping Zheng
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Mice were anesthetized with ketamine and xylazine (160 mg/kg and 12 mg/kg body weight, respectively) before the stereotaxic surgery was performed. For retrograde labeling experiments, mice received bilateral injections (0.5 μl in each side, infused over 10 min) of FG (Fluorochrome, USA) into the PrL [anteroposterior (AP), +1.98 mm; mediolateral (ML), ±0.30 mm; dorsoventral (DV), −2.20 mm]. For in vivo optogenetic inhibition in CPA experiments, mice were injected with the AAV8-CaMKIIα-eNpHR-eYFP virus or the same viral vectors carrying eYFP alone (2.45 × 1012 vector genomes/ml; Neuron Biotech Company, China) bilaterally into the BLA (AP, −1.60 mm; ML, ±3.35 mm; DV, −4.80 mm) or the PrL at a volume of 0.5 μl for 10 min. To allow projection-specific targeting, the optical fiber was held at least 500 μm above eYFP-expressing axonal terminals. For all the above stereotaxic injections, the needle was retained in place for an additional 10 min to allow diffusion of the injected solutions.

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