2.4. Peptide release from the particles

The amount of peptide released from peptide-encapsulated particles was investigated, with 10 mg of PLG particles washed, resuspended in 1 ml PBS, and incubated at 37 °C with agitation. The in vitro release of PLP_139–151 from agPLG-L, agPLA and agPLG-H particles was measured at several time points during incubation in PBS at 37 °C for 456 h (19 days). At indicated time points, particles were centrifuged, and the supernatant was collected and replaced with fresh PBS. Peptide in the supernatant was measured using the Micro BCA Protein Assay Kit. Cumulative release and release at each time point was calculated as percent of the amount of peptide detected divided by total amount of encapsulated peptide.