2.7. Ovarian Cancer Cell Invasion Assay

In 24-well plates (Corning, NY, USA), three lines of ovarian cancer cells (5 × 105) were seeded in Transwell chambers placed. The 8 μm pore membranes in the 24-well plates were covered with 50 μL of a 1 : 10 dilution of Matrigel™ matrix. Cancer cells were cultured on the membranes for 6 h. Two types of medium were infused into the lower chamber: ADSC CM supplemented with 10% FBS or normal culture medium supplemented with 10% FBS (control). Transwell assays were performed while culturing ovarian cancer cells without FBS for one day. Next, 4% paraformaldehyde was used to fix all cancer cells. Cancer cells that penetrated through the membranes were analysed. The number of cells that migrated through the membrane in each high magnification field was calculated. Every experiment was performed for three times.