Proteasome inhibition assay

XW Xunwei Wu
AV Alice Tommasi di Vignano
QZ Qian Zhou
PM Piotr J. Michel-Dziunycz
FB Fuxiang Bai
JM Jun Mi
JQ Jing Qin
TZ Tingjian Zu
GH Günther F. L. Hofbauer
request Request a Protocol
ask Ask a question
Favorite

To duplicate 60 mm dishes, either 1 μM Epoxomicin (#24801, EMD), 10 μM MG132 or vehicle (DMSO) was added directly to the cell medium, starting 3 hr after UVB treatment and subsequently after an additional 2 and 5 hr. Cells were collected at the indicated times and then lysed in loading buffer for Western blot analysis.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A