2.5. Growth Factor Gradient Generation for Chemotaxis Experiment

For chemotaxis experiments, 100 µL of cell medium was introduced into the proximal chamber where myoblasts were seeded, while 100 µL cell medium supplemented with 11 ng/mL HGF or 11 ng/mL BFGF or 10 μM FITC-conjugated dextran was introduced in to the distal chamber. For growth factor experiments, after a 24 h period, 2 µM Calcein AM was introduced in both chambers and cells were imaged as described below. For the visualization of the gradient generated by FITC molecules, images were acquired of both chambers every 30 min over an 8 h period.