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Generation of polyclonal antibodies

SK Seth D. Kasowitz
JM Jun Ma
SA Stephen J. Anderson
NL N. Adrian Leu
YX Yang Xu
BG Brian D. Gregory
RS Richard M. Schultz
PW P. Jeremy Wang
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The GST-YTHDC1 (aa 3–109) fusion protein (S2A Fig) was expressed in E. coli using the pGEX4T-1 vector and affinity purified with glutathione Sepharose 4B (GE Healthcare). Rabbits were immunized with recombinant protein, yielding antisera UP2410 and UP2411 (Cocalico Biologicals Inc.). For western blotting and immunofluorescence, antibodies were affinity purified against the GST fusion protein.

Copyright and License information:  ©2018 Kasowitz et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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