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2.4. Assessment of Bacterial Membrane Properties by Flow Cytometry
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After incubation time, supernatants were flushed several times and 50 μL was collected for staining in 200 μL PBS containing propidium iodide (Sigma-Aldrich) and Syto-62© (Life Technologies) at final concentration of, respectively, 20 μM and 1 μM. For assessing membrane potential, supernatants were stained with 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3), 1 μm, Sigma-Aldrich) [14] and Syto-62©. All experiments were analyzed by flow cytometry using FACSCalibur (BD Biosciences, Le Pont de Claix, France).
Copyright and License information: ©2019 Paul Monsarrat et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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