Antibodies and reagents

The following primary antibodies were used: anti-NDRG1 rabbit monoclonal antibody (Abcam, ab124689), anti-PCNA(P10) mouse monoclonal antibody (CST, #2586), anti-PCNA rabbit polyclonal antibody (ABclonal, A4006), anti-α-tubulin antibody (Sigma, T6199), anti-FLAG antibody (Sigma, F1804), anti-CTCF mouse monoclonal antibody (Santa Cruz, sc-271514), anti-CTCF rabbit polyclonal antibody (ABclonal, A1133), anti-LANA rat monoclonal antibody (Advanced Biotechnology Inc, 13-210-1000), and anti-LANA mouse monoclonal antibody (1B5), which was prepared in our laboratory [73]. The secondary antibodies used for western blotting and the IF assay were goat anti-mouse IRDye 800CW (Li-Cor, 926–32210); goat anti-rabbit IRDye 680RD (Li-Cor, 926–68071); goat anti-rabbit antibodies conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, A-11094), 555 (Thermo Fisher Scientific, A27017), and 680 (Thermo Fisher Scientific, {"type":"entrez-protein","attrs":{"text":"A27020","term_id":"84105","term_text":"pir||A27020"}}A27020); goat anti-mouse antibodies conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, A-11001) and 555 (Thermo Fisher Scientific, A-21422); and goat anti-rat antibodies conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, A-11006). The other reagents used (and their sources) were as follows: anti-FLAG M2 affinity gel (Sigma, A2220), Strep-Tactin Sepharose (IBA, 2-1201-010), desthiobiotin (IBA, 2-1000-001), recombinant protein A agarose (Invitrogen, 15948–014), recombinant protein G agarose (Invitrogen, 15920–010), glutathione Sepharose 4B (GE Healthcare, 17-0756-01), the TNT T7 Quick Coupled transcription/translation system (Promega L1170), Dynabeads M-280 streptavidin (Invitrogen, 11205D).