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Fasting morning blood samples were taken, stored at −80°C, and analyzed at the end of the study. For patients on hemodialysis, blood samples were taken on the day after their hemodialysis session. We measured serum iPTH, bALP, intact PINP, CTX, TRAP5b, and 25-hydroxyvitamin D using the IDS-iSYS autoanalyzer (Immunodiagnostic Systems). Total PINP was measured using the Cobas e411 automated immunoassay (Roche Diagnostics). We measured fasting serum calcium, phosphate, total alkaline phosphatase, and creatinine on a Roche Cobas c701/702 analyzer (Roche Diagnostics) on the same day as sample collection. eGFR was calculated using the Modification of Diet in Renal Disease equation.
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