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4.2. Oxygen Glucose Deprivation/Reperfusion (OGD/R) of Primary Cortical Neurons

RH Rongrong Hua
SH Song Han
NZ Nan Zhang
QD Qingqing Dai
TL Ting Liu
JL Junfa Li
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The OGD/R model was used to simulate ischemia/reperfusion injury in vitro. As described above, a portion of the neurons cultured for eight days were subjected to OGD/R [21]. The terms of OGD treatment were glucose-free DMEM (11966-025, Gibco, Grand Island, USA) and 5% CO2/2% O2/93% N2 for 1 h. The terms of reperfusion treatment were Neurobasal Medium, which contained 2% B27 supplement and 5% CO2/21% O2/74% N2 for 1, 3, 6, 9, or 12 h, respectively.

Copyright and License information:  ©2018 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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