4.4. Lactate Dehydrogenase (LDH) Release Assay

B16F10 cells were seeded in a 24-well plate and cultured 24 h and incubated with various concentrations of tobramycin (8 and 16 mg/mL) for 72 h. After incubation, 10 μL of the culture supernatant was transferred into a new 96-well plate. LDH released to the extracellular medium was quantified by the EZ-LDH cell cytotoxicity assay kit according to the manufacturer’s instructions. Fresh culture medium was used as blank. The absorbance was measured at 450 nm using an ELISA reader.