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Measurement of c-peptide-positive cells
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The cellular aggregates formed by SC were dispersed into a single-cell suspension by incubation in TrypLE Express (Thermo Fisher Scientific) at 37°C, then 3 μL of the cell suspension at a concentration of 2.5 × 107 cells/mL were smeared using Smear Gell (Genostaff), according to the manufacturer’s protocol. Subsequently, c-peptide immunostaining was performed, and the rate of c-peptide-positive cells was determined by manually counting 50 random fields at 200× magnification in 5 independent samples.
Copyright and License information: ©2018 Motoyama et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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