Bioinformatic Analysis Based on Data in the TCGA

P4HA3 RNA expression in gastrointestinal and some other solid tumors and in the corresponding normal tissues was examined using data from TCGA. In this database, 478 patients with GC having primary tumors, and 102 cases of normal solid tissues were included. Four hundred fifteen GC samples and 32 normal samples were subjected to RNA-seq analysis (IlluminaHiSeq UNC). The inclusion of patients and the availability of the genomic and pathological data were summarized in Supplemental Figure 1. Data analysis was performed using the FireBrowse (http://firebrowse.org/). Heatmap of P4HA3 exon expression in patients with primary GC was reviewed using the UCSC Xena browser (https://xenabrowser.net/). The correlation between P4HA3 RNA and SNAI2 RNA expression was analyzed. Kaplan-Meier plots of 5-year and 10-year OS were generated by dividing the patients into high and low P4HA1/P4HA2/P4HA3 expression groups according to median exon expression. The genes coexpressed with P4HA3 in TCGA-Stomach Cancer (STAD) were identified by using cBioPortal for Cancer Genomics.^16,17