Animals

A total of 30 Wistar male rats were used (2 months old) in each round of experiments, kindly provided by the Bioterium of the Instituto de Investigaciones Biomédicas (IIB), Universidad Nacional Autónoma de Mexico (UNAM). The animals were organized into six groups and allocated in polycarbonate boxes (50 cm l × 23 cm w × 21 cm h). The groups were distributed as follows: (i) untreated control (Int-Ctrl n=5), (ii) untreated infected (Int-Infx n=5), (iii) simulated adenohypophysis implant in renal capsule surgery (Sham) control (Sh-HPRL-Ctrl n=5), (iv) simulated adenohypophysis implant in renal capsule surgery (Sham) infected (Sh-HPRL-Infx n=5), (v) adenohypophysis implant in renal capsule surgery control (HPRL-Ctrl n=5), and (vi) adenohypophysis implant in renal capsule surgery infected (HPRL-Infx n=5). Two rounds of experiments were performed. Animals were kept in cycles of 12 h of light/darkness. Water and food (Harlan 2019S Teklad Global 19% Protein Extruded Rodent Diet Sterilizable) were supplied ad libitum in sterile conditions.