Restriction Digest of Phage Genomes

For restriction digest of phage genomes, phages were propagated on host strains and purified and harvested by filtration (0.22 μm pore size), followed by concentration using VIVASPIN^® 15 10,000MWCO PES membranes (Sartorius). Concentrated phage lysate (1 mL) was treated with 10 μg DNAse I (Sigma-Aldrich) and 5 μg RNase A (Sigma-Aldrich) for 30 min at 37°C, prior to extraction of phage DNA using the QIAmp Min Elute Virus Spin kit (QIAGEN). Recovered phage DNA was digested using EcoRI and XbaI individually, according to manufacturer’s instructions (Fermentas Fast Digest^® enzymes), and restriction fragments separated using agarose gel electrophoresis.