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Electron Microscopy

DA Diana R. Alves
JN Jonathan Nzakizwanayo
CD Cinzia Dedi
CO Chara Olympiou
AH Aurélie Hanin
WK Witold Kot
LH Lars Hansen
RL Rene Lametsch
CG Cormac G. M. Gahan
PS Pascale Schellenberger
LO Lesley A. Ogilvie
BJ Brian V. Jones
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Purified phage particles at a concentration of 109 PFU/mL were deposited on 300 mesh carbon-coated copper grids (Agar Scientific, United Kingdom) and negatively stained with 1% uranyl acetate (pH 4) as follows: the surface of the copper grids were ionized for 2 min immediately prior to sample deposition. 5 μL of phage lysate were spotted on the surface of the grid and allowed to stand for 1 min. This was followed by 30 sec negative staining and subsequent air drying of the grid. Visualization was performed using a JEOL JEM-1400Plus TEM, operated at 120 kV (pixel size = 0.1 nm), equipped with a Gatan OneView 4K camera with automatic drift correction.

Copyright and License information:  ©2019 Alves, Nzakizwanayo, Dedi, Olympiou, Hanin, Kot, Hansen, Lametsch, Gahan, Schellenberger, Ogilvie and Jones.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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