Monoclonal Antibodies.

IFN-a blocking monoclonal antibodies against the IFNAR1 receptor (clone: MAR1–5A3) were purchased from BioXcell, and 250–500 μg (loading dose 500 μg followed by 250 μg twice weekly) was administered in the peritoneum (ip). Anti-PD-L1 (clone: 10F.9G2) and anti-PD-1 monoclonal antibodies (clone: RMP1–14) were purchased from BioXcell, and 200–400 μg/dose (loading dose 400 μg followed by 200 μg twice weekly) was administered in the peritoneum of C57Bl/6 mice. PDCA-1 depleting mAbs (clone: 120G8.04) were purchased from Novus Biologicals, and 200–250 μg was administered in the peritoneum of C57Bl/6 mice (1 day before RNA-NP administration).