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Excess NAC and other impurities present in the solution containing NAC coated Nano-ZnO product was removed by dialysis (Spectrum Labs, Spectra/Por 3 standard RC dry dialysis tubing, MWCO 3.5 kD). Dialysis was carried out for 72 hours changing DI water in every 8 hours. Nearly 300 mg of purified NAC coated Nano-ZnO powder was obtained after freeze-drying the entire dialyzed (initial volume of 25 ml) solution. Hereafter, the NAC coated Nano-ZnO was abbreviated as Nano-ZnO.
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