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Cell culture and activation

JY Jing Yu
ZL Zaizhou Liu
YL Yuanyuan Liang
FL Feng Luo
JZ Jie Zhang
CT Cuiping Tian
AM Alex Motzik
MZ Mengmeng Zheng
JK Jingwu Kang
GZ Guisheng Zhong
CL Cong Liu
PF Pengfei Fang
MG Min Guo
ER Ehud Razin
JW Jing Wang
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RBL-2H3 cells were obtained from the cell bank of the Chinese Academy of Science (catalog number TCR7), cultured and maintained at 37 °C in growth medium containing RPMI, 2 mM l-glutamine, 2 mM nonessential amino acids, 100 units ml−1 penicillin, 100 μg ml−1 streptomycin, and 10% fetal bovine serum. For IgE and antigen activation, RBL-2H3 cells were first treated with mouse-IgE antibody (Sigma-Aldrich, catalog number D8406) at 400 ng ml−1 for 2 h at 37 °C. Followed by 3× wash with warm phosphate-buffered saline (PBS) buffer, cells were stimulated with 400 ng ml−1 DNP-albumin (Sigma-Aldrich).

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