Data availability statement

Strains and other reagents are available from the Caenorhabditis Genetics Center or upon request. The authors affirm that all data necessary for confirming the conclusions of the article are present within the article, figures, and table, together with supplementary Tables and Figures that have been uploaded to figshare. Figure S1 contains progenitor zone counts relevant to Figures 1 and ​and2.2. Figure S2 is a workflow diagram for the primary RNAi screen. Figure S3 shows the distribution of predicted positive wells from the screen across linkage groups, selection criteria for the 2^nd pass of the primary screen (including examples), and a Venn diagram of the clones selected from the 1^st pass. Figure S4 presents the genomic scenario and analysis for two genes (rpl-24.2 and C03D6.1) that were targeted by a single clone from the Ahringer RNAi Library. Table S1 provides details on strains, alleles and plasmids used. Table S2 contains raw data from the primary screen. Table S3 lists the set of 133 genes, their mammalian ortholog(s) and disease associations, their distribution into the sets of 77 and 56 genes, and information on the status of the progenitor pool when each was depleted by RNAi. Table S4 lists the set of 77 genes and whether or not they were found in 7 other C. elegans screens. Table S5 displays in 3 tabs, the PANTHER representation analysis of the sets of 133 and 56 genes by biological process, cellular compartment, and molecular function. Table S6 shows all p- and n-values for Figures 1A, 1C, 1D, 2, 6, S1, and S4. Supplemental material available at Figshare: https://doi.org/10.25387/g3.6869828.