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DNA sequencing

FM Fernanda C. P. Mesquita
PA Paulo C. Arantes
TK Tais H. Kasai-Brunswick
DA Dayana S. Araujo
FG Fernanda Gubert
GM Gustavo Monnerat
DS Danúbia Silva dos Santos
GN Gabriel Neiman
IL Isabela C. Leitão
RB Raiana A. Q. Barbosa
JC Jorge L. Coutinho
IV Isadora M. Vaz
MS Marcus N. dos Santos
TB Tamara Borgonovo
FC Fernando E. S. Cruz
SM Santiago Miriuka
EM Emiliano H. Medei
AC Antonio C. Campos de Carvalho
AC Adriana B. Carvalho
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Genomic DNA was extracted from the iPSCs and exons corresponding to the coding region of KCNH2 were amplified as previously published26. To amplify exon 7 of KCNH2, the PCRx Enhancer Reagent (Thermo Scientific) was added to the reaction, according to the manufacturer’s instructions. PCR products were purified by ExoSAP-IT PCR Product Cleanup Reagent (Affymetrix) and sequenced using sense or antisense primers, according to manufacturer’s instructions (primer sequences are available in Supplementary Table 1) in 3500-xL Genetic Analyzer with BigDye terminator v3.1 Cycle Sequencing Kit (Thermo Scientific).

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